Review

human il 6 elisa duoset kit (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems human il 6 elisa duoset kit

( a ) Cell viability was assessed with the MTT assay and data shown are means ± SD (n = 3). ( b ) <t>TNFα,</t> <t>IL-6</t> and IL-8 expression in response to B[a]P exposure. ( c ) Representative Western blot images showing protein expression. All data were obtained from three independent experiments. Statistical p values were determined by a one-way ANOVA, followed by a Tukey post-hoc test comparing the B[a]P treatments to the DMSO control; * p < 0.05; ¥, p < 0.01; NS, no significance.

Human Il 6 Elisa Duoset Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+6+duoset+elisa/Human+IL-8%2FCXCL8+DuoSet+ELISA/bio_rxiv__64898__2026__04__21__719931-251-60-70
Average 96 stars, based on 647 article reviews

human il 6 elisa duoset kit - by Bioz Stars, 2026-10

96/100 stars

Images

1) Product Images from "PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling"

Article Title: PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling

Journal: bioRxiv

doi: 10.64898/2026.04.21.719931

( a ) Cell viability was assessed with the MTT assay and data shown are means ± SD (n = 3). ( b ) TNFα, IL-6 and IL-8 expression in response to B[a]P exposure. ( c ) Representative Western blot images showing protein expression. All data were obtained from three independent experiments. Statistical p values were determined by a one-way ANOVA, followed by a Tukey post-hoc test comparing the B[a]P treatments to the DMSO control; * p < 0.05; ¥, p < 0.01; NS, no significance.

Figure Legend Snippet: ( a ) Cell viability was assessed with the MTT assay and data shown are means ± SD (n = 3). ( b ) TNFα, IL-6 and IL-8 expression in response to B[a]P exposure. ( c ) Representative Western blot images showing protein expression. All data were obtained from three independent experiments. Statistical p values were determined by a one-way ANOVA, followed by a Tukey post-hoc test comparing the B[a]P treatments to the DMSO control; * p < 0.05; ¥, p < 0.01; NS, no significance.

Techniques Used: MTT Assay, Expressing, Western Blot, Control

Cells were treated with various concentrations of PM2.5 for 24 hr. ( a ) Cell viability was assessed with MTT assay. ( b ) IL-6 and IL-8 from the basolateral sides. ( c ) Representative Western blot images showing CFTR, β2AR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), NF-κB p65, and TRPC6 expression. All data are presented as the mean ± SD (n = 4). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; *, p < 0.05; ¥, p < 0.01; NS, no significance.

Figure Legend Snippet: Cells were treated with various concentrations of PM2.5 for 24 hr. ( a ) Cell viability was assessed with MTT assay. ( b ) IL-6 and IL-8 from the basolateral sides. ( c ) Representative Western blot images showing CFTR, β2AR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), NF-κB p65, and TRPC6 expression. All data are presented as the mean ± SD (n = 4). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; *, p < 0.05; ¥, p < 0.01; NS, no significance.

Techniques Used: MTT Assay, Western Blot, Expressing, Control

( a ) IL-6 and IL-8 from the basolateral sides of the ALI were determined using the DuoSet® ELISA kits. Data are presented as the mean ± SD (n = 4). Statistical p -values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the all treatments to the DMSO control; ¥, p < 0.05; *, p < 0.01. ( b ) Representative Western blot images showing CFTR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), and NF-κB p65 expression in treated polarized 16HBE14o-cells in ( a ). All data were obtained from three independent experiments.

Figure Legend Snippet: ( a ) IL-6 and IL-8 from the basolateral sides of the ALI were determined using the DuoSet® ELISA kits. Data are presented as the mean ± SD (n = 4). Statistical p -values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the all treatments to the DMSO control; ¥, p < 0.05; *, p < 0.01. ( b ) Representative Western blot images showing CFTR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), and NF-κB p65 expression in treated polarized 16HBE14o-cells in ( a ). All data were obtained from three independent experiments.

Techniques Used: Enzyme-linked Immunosorbent Assay, Control, Western Blot, Expressing

ALI cells were treated with DMSO, 100 ng/mL PM 2.5, 10 µM LY 294002 ± PM 2.5, or 10 µM LY 303511 ± PM 2.5 for 24 hrs. ( a ) IL-6 and IL-8 from the basolateral sides were determined using the DuoSet® ELISA kits. Data are presented as the mean ± SD (n=3). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing all treatments to the DMSO control, or PM 2.5 to PM 2.5 ± LY 294002 or LY 303511; *, p < 0.01. ( b ) Representative Western blot images showing CFTR and β-actin expression. β-actin was used to ensure equal loading of protein. All data were obtained from three independent experiments, and each experiment was analyzed in duplication.

Figure Legend Snippet: ALI cells were treated with DMSO, 100 ng/mL PM 2.5, 10 µM LY 294002 ± PM 2.5, or 10 µM LY 303511 ± PM 2.5 for 24 hrs. ( a ) IL-6 and IL-8 from the basolateral sides were determined using the DuoSet® ELISA kits. Data are presented as the mean ± SD (n=3). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing all treatments to the DMSO control, or PM 2.5 to PM 2.5 ± LY 294002 or LY 303511; *, p < 0.01. ( b ) Representative Western blot images showing CFTR and β-actin expression. β-actin was used to ensure equal loading of protein. All data were obtained from three independent experiments, and each experiment was analyzed in duplication.

Techniques Used: Enzyme-linked Immunosorbent Assay, Control, Western Blot, Expressing

( a ) The lung parenchyma from two ferrets were sliced into several sections as shown. Arrows indicate the tracheobronchial tubes. All tissue sections were incubating with DMSO (control), 10 µM B[a]P, or 100 ng/mL PM 2.5 in Pneumacult-EX Plus Basal media for 24 hrs. ( b ) IL 6 secretion was determined using the ferret ELISA kit. Data represent the mean of 6 independent sections. Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; ¥, p < 0.05 (n = 6). ( c ) Representative western blotting images show reduction of CFTR, and β2AR and elevation of TRPC6 expression in explants under these treatments.

Figure Legend Snippet: ( a ) The lung parenchyma from two ferrets were sliced into several sections as shown. Arrows indicate the tracheobronchial tubes. All tissue sections were incubating with DMSO (control), 10 µM B[a]P, or 100 ng/mL PM 2.5 in Pneumacult-EX Plus Basal media for 24 hrs. ( b ) IL 6 secretion was determined using the ferret ELISA kit. Data represent the mean of 6 independent sections. Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; ¥, p < 0.05 (n = 6). ( c ) Representative western blotting images show reduction of CFTR, and β2AR and elevation of TRPC6 expression in explants under these treatments.

Techniques Used: Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Enzyme-linked Immunosorbent Assay:

Article Title: Microphysiological System-Generated Physiological Shear Forces Reduce TNF-α-Mediated Cartilage Damage in a 3D Model of Arthritis.
Article Snippet: .. Enzyme-Linked Immunosorbent Assay (ELISA): TNF-α and IL-6 DuoSet ELISA were performed according to the manufacturer’s instructions in duplicates (R&D Systems, Minneapolis, USA). .. Briefly, Nunc MaxiSorp high protein-binding capacity 96-well flat-bottomed plates (Thermo Fisher Scientific, Waltham, USA) were incubated overnight at RT with the capture antibody.

Article Title: Chronic consumption of a hypercaloric diet increases neuroinflammation and brain senescence, promoting cognitive decline in middle-aged female Wistar rats
Article Snippet: .. TNF-α DuoSet ELISA (DY510), IL-6 DuoSet ELISA (DY506), IL-10 DuoSet ELISA (DY522), IL-1β DuoSet ELISA (DY501), MCP-1 DuoSet ELISA (DY3144), BDNF DuoSet ELISA (DY248), and DuoSet ELISA Ancillary Reagent Kit (DY008), all purchased from R&D Systems (Minneapolis, MN, United States). .. All required solutions were prepared with deionized water from a Milli-RQ system (Millipore, MA).

Article Title: Caspase-11 regulates systemic inflammation and cell death in a cell-specific manner after trauma with shock
Article Snippet: Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were detected by the DRI-CHEM 4000 Chemistry Analyzer System (Heska) according to the manufacturer's instructions. .. Cytokine abundance was measured by ELISA according to the protocol with mouse IL-10 DuoSet ELISA, IL-6 DuoSet ELISA, IL-1β, CXCL-1/KC DuoSet ELISA from R&D Systems. .. Cystatin C ELISA Kit Quantikine (R&D System, MSCTC0).

Article Title: Microphysiological System‐Generated Physiological Shear Forces Reduce TNF‐α‐Mediated Cartilage Damage in a 3D Model of Arthritis
Article Snippet: .. TNF‐α and IL‐6 DuoSet ELISA were performed according to the manufacturer's instructions in duplicates (R&D Systems, Minneapolis, USA). .. Briefly, Nunc MaxiSorp high protein‐binding capacity 96‐well flat‐bottomed plates (Thermo Fisher Scientific, Waltham, USA) were incubated overnight at RT with the capture antibody.

Article Title: Construction of pVAX-1-based linear covalently closed vector with improved transgene expression.
Article Snippet: 1 Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu 625021, India 2 Centre for Advanced Biotherapeutic and Regenerative Medicine, Faculty of Research, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, India 3 Research and Collaboration, Anka Analytica, Melbourne, Australia 4 External Consultant, Independent Researcher, Pittsfield, MA, USA 5 Centre for Herbal Pharmacology and Environmental Sustainability, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, India Abstract Introduction This study presents a Mammalian Linear Expression System (MLES), a linear covalently closed (LCC) vector based on pVAX-1.. The purpose of this system was to improve gene expression in mammalian cells and to test the efficacy of MLES in transient transfection and transgene expression using in vitro and in vivo models.. Additionally, we aimed to evaluate potential inflammatory responses in vivo.

Article Title: A novel TIRAP-MyD88 inhibitor blocks TLR7- and TLR8-induced type I IFN responses.
Article Snippet: Other cytokines for human primary cells were analyzed using BioPlex cytokine assays from Bio-Rad, in accordance with the instructions of the manufacturer with the recommended concentration of reagents, but in reduced volume (1:2), using the Bio-Plex Pro Reagent Kit III and BioPlex 200 System (Bio-Rad). .. TNF, IL-6, and IL-8 ELISA were used instead of Bio-Plex when indicated in the figure legends and were human TNF-alpha DuoSet ELISA (#DY210), IL-6 DuoSet ELISA (#DY206), and IL-8/CXCL8 Duoset ELISA (#DY208) (R&D Systems). .. After addition of 20 μM peptide or solvent (water, H2O) for 30 min, samples were stimulated with LPS (100 ng/mL), CL075, R837 (1 μg/mL) or 2 × 106 of pHrodo Red E. coli (#P35361; Thermo Fisher Scientific) or 4 × 106 of pHrodo Red Staphylococcus aureus (#A10010; Thermo Fisher Scientific) bioparticles for 5 h at 37 �C (slow rotation).

Article Title: The prohibitin-binding compound fluorizoline induces the pro-inflammatory cytokines interleukin-8 and interleukin-6 through the activation of JNK and p38 MAP kinases.
Article Snippet: .. IL-8 and IL-6 levels in the cell culture supernatants were measured with a quantitative enzyme-linked immunosorbent assay according to each manufacturer’s procedures. (Human IL-8/CXCL8 or IL-6 DuoSet ELISA; #DY208 and #DY206, respectively; R&D Systems). ..

other:

Article Title: Suckerin Colloids and Hydrogels With Low Immunogenicity as Resorbable and Hemostatic Tissue Adhesives for Wound Healing.
Article Snippet: Squid sucker ring teeth proteins (suckerins) have recently been shown to exhibit higher molecular-level adhesion than mussel adhesive proteins–a well-known class of wet-resistant adhesive proteins–and can also assemble into nanoparticles in aqueous conditions.. Herein, we present recombinant suckerin-12 colloids and hydrogels as bioadhesives with strong wet adhesion and excellent in vivo biocompatibility.. These colloids and hydrogels displayed low cytotoxicity, minimal immunogenicity, and negligible hemolysis, while outperforming fibrin glue in hemostasis, skin cell proliferation and migration, wound closure, and healing.

In Vivo:

Cell Culture:

Image Search Results

Journal: bioRxiv

Article Title: PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling

doi: 10.64898/2026.04.21.719931

Figure Lengend Snippet: ( a ) Cell viability was assessed with the MTT assay and data shown are means ± SD (n = 3). ( b ) TNFα, IL-6 and IL-8 expression in response to B[a]P exposure. ( c ) Representative Western blot images showing protein expression. All data were obtained from three independent experiments. Statistical p values were determined by a one-way ANOVA, followed by a Tukey post-hoc test comparing the B[a]P treatments to the DMSO control; * p < 0.05; ¥, p < 0.01; NS, no significance.

Article Snippet: Benzo[a]pyrene, PM 2.5 (ERMCZ110), CFTR inh -172, IBMX, amiloride, Forskolin, MG-132, L-glutathione, and Millipore Immobilon Western (Sigma-Aldrich); BI 749327, LY 294002, and LY 303511 (Cayman Chemical); MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide; EZ-LinkTM Sulfo-NHS-SS-Biotin, CellROX green reagent, Dynabeads Protein G, Pierce Streptavidin magnetic beads, HaltTM protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) ; Fluo-4 AM and Probenecid (Invitrogen); Human IL-8/CXCL8 DuoSet® ELISA kit, Human IL-6 ELISA DuoSet® kit, and Human TNF-alpha DuoSet® ELISA (R&D Systems); Ferret IL-6 ELISA kit (Genorise, PA, USA); Pneumacult-ALI media kit and Pneumacult-EX Plus Basal media kit (Stemcell); and Gibco MEM (Thermo Fisher).

Techniques: MTT Assay, Expressing, Western Blot, Control

Journal: bioRxiv

Article Title: PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling

doi: 10.64898/2026.04.21.719931

Figure Lengend Snippet: Cells were treated with various concentrations of PM2.5 for 24 hr. ( a ) Cell viability was assessed with MTT assay. ( b ) IL-6 and IL-8 from the basolateral sides. ( c ) Representative Western blot images showing CFTR, β2AR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), NF-κB p65, and TRPC6 expression. All data are presented as the mean ± SD (n = 4). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; *, p < 0.05; ¥, p < 0.01; NS, no significance.

Techniques: MTT Assay, Western Blot, Expressing, Control

Journal: bioRxiv

Article Title: PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling

doi: 10.64898/2026.04.21.719931

Figure Lengend Snippet: ( a ) IL-6 and IL-8 from the basolateral sides of the ALI were determined using the DuoSet® ELISA kits. Data are presented as the mean ± SD (n = 4). Statistical p -values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the all treatments to the DMSO control; ¥, p < 0.05; *, p < 0.01. ( b ) Representative Western blot images showing CFTR, phospho-AKT (Ser473), AKT, phospho-NF-κB p65 (Ser536), and NF-κB p65 expression in treated polarized 16HBE14o-cells in ( a ). All data were obtained from three independent experiments.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Western Blot, Expressing

Journal: bioRxiv

Article Title: PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling

doi: 10.64898/2026.04.21.719931

Figure Lengend Snippet: ALI cells were treated with DMSO, 100 ng/mL PM 2.5, 10 µM LY 294002 ± PM 2.5, or 10 µM LY 303511 ± PM 2.5 for 24 hrs. ( a ) IL-6 and IL-8 from the basolateral sides were determined using the DuoSet® ELISA kits. Data are presented as the mean ± SD (n=3). Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing all treatments to the DMSO control, or PM 2.5 to PM 2.5 ± LY 294002 or LY 303511; *, p < 0.01. ( b ) Representative Western blot images showing CFTR and β-actin expression. β-actin was used to ensure equal loading of protein. All data were obtained from three independent experiments, and each experiment was analyzed in duplication.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Western Blot, Expressing

Journal: bioRxiv

Article Title: PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling

doi: 10.64898/2026.04.21.719931

Figure Lengend Snippet: ( a ) The lung parenchyma from two ferrets were sliced into several sections as shown. Arrows indicate the tracheobronchial tubes. All tissue sections were incubating with DMSO (control), 10 µM B[a]P, or 100 ng/mL PM 2.5 in Pneumacult-EX Plus Basal media for 24 hrs. ( b ) IL 6 secretion was determined using the ferret ELISA kit. Data represent the mean of 6 independent sections. Statistical p values were determined with a one-way ANOVA, followed by the Tukey post-hoc test comparing the B[a]P or PM 2.5 treatments to the DMSO control; ¥, p < 0.05 (n = 6). ( c ) Representative western blotting images show reduction of CFTR, and β2AR and elevation of TRPC6 expression in explants under these treatments.

Techniques: Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

a, Violin plots showing expression of Prg4 , Thy1 and Cd34 across fibroblast clusters. b, Heatmap of top 20 DEGs defining lining fibroblasts (LFs : Prg4 ⁺) and five sublining fibroblast populations (SLFs : Myoc ⁺, Ugdh ⁺, Cxcl12 ⁺, Col3a1 ⁺ and Ccl11 ⁺). c, Violin plots showing expression of representative marker genes across fibroblast subtypes. d, UMAPs showing fibroblast clusters in young and aged mice, with relative cluster proportions by age group. e, Gene Ontology enrichment analysis of genes upregulated in young versus aged fibroblast clusters. Dot size indicates gene ratio and color indicates adjusted P value. f, CellChat analysis of IL-6 signaling networks in young and aged synovium, showing inferred communication probabilities among cell populations. g, SenMayo module scores across fibroblast clusters.

Journal: bioRxiv

Article Title: Single-cell atlas reveals age-related cellular shifts underlying fibrosis in murine synovium

doi: 10.64898/2026.04.17.719179

Figure Lengend Snippet: a, Violin plots showing expression of Prg4 , Thy1 and Cd34 across fibroblast clusters. b, Heatmap of top 20 DEGs defining lining fibroblasts (LFs : Prg4 ⁺) and five sublining fibroblast populations (SLFs : Myoc ⁺, Ugdh ⁺, Cxcl12 ⁺, Col3a1 ⁺ and Ccl11 ⁺). c, Violin plots showing expression of representative marker genes across fibroblast subtypes. d, UMAPs showing fibroblast clusters in young and aged mice, with relative cluster proportions by age group. e, Gene Ontology enrichment analysis of genes upregulated in young versus aged fibroblast clusters. Dot size indicates gene ratio and color indicates adjusted P value. f, CellChat analysis of IL-6 signaling networks in young and aged synovium, showing inferred communication probabilities among cell populations. g, SenMayo module scores across fibroblast clusters.

Article Snippet: Mouse TNFα (DY410, R&D Systems) and IL-6 (DY406, R&D Systems) concentrations in serum samples and cell culture supernatants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Expressing, Marker

a , Serum IL-6 levels in young and aged male mice (n = 8 per group). b , Representative flow cytometry gating strategy for peripheral blood myeloid cells. Bar graphs show the quantification of Ly6G + neutrophils, Ly6C high monocytes and Ly6C low monocytes per ml of blood in young and aged male mice (n = 9 per group). c , Representative flow cytometry gating strategy for synovial tissue monocytes and neutrophils. Flow cytometry-based enumeration of Ly6C + monocytes and Ly6G + neutrophils per paw in young and aged male mice (n = 6 per group). Data are mean ± s.e.m. Two-tailed Mann-Whitney U test. * P < 0.05, *** P < 0.001; ns, not significant.

Journal: bioRxiv

Article Title: Single-cell atlas reveals age-related cellular shifts underlying fibrosis in murine synovium

doi: 10.64898/2026.04.17.719179

Figure Lengend Snippet: a , Serum IL-6 levels in young and aged male mice (n = 8 per group). b , Representative flow cytometry gating strategy for peripheral blood myeloid cells. Bar graphs show the quantification of Ly6G + neutrophils, Ly6C high monocytes and Ly6C low monocytes per ml of blood in young and aged male mice (n = 9 per group). c , Representative flow cytometry gating strategy for synovial tissue monocytes and neutrophils. Flow cytometry-based enumeration of Ly6C + monocytes and Ly6G + neutrophils per paw in young and aged male mice (n = 6 per group). Data are mean ± s.e.m. Two-tailed Mann-Whitney U test. * P < 0.05, *** P < 0.001; ns, not significant.

Techniques: Flow Cytometry, Two Tailed Test, MANN-WHITNEY

a , Heatmap showing expression of top 20 genes across synovial macrophage clusters, identifying Pf4 + , Vsig4 + and Cd74 + macrophage subsets. b , Violin plots depict expression of representative marker genes across the three macrophage subsets. c , Gene Ontology enrichment analysis of genes upregulated in young versus aged macrophages within each subset. Dot size indicates gene ratio and color indicates adjusted P value. d , IL-6 and TNF-α secretion by FACS-sorted synovial macrophages from young and aged mice following 24 h ex vivo stimulation with or without LPS (n = 10 mice per group; one-way ANOVA test). e , Phagocytosis assay showing representative pHrodo Green fluorescence histograms and frequency of pHrodo + cells within CD64 + F4/80 + synovial macrophages, 2 h after intra-articular injection of pHrodo Green E. coli BioParticles (n = 6 mice per group; two-tailed Mann-Whitney U test). FMO control is shown in grey. Data are mean ± s.e.m. ** P < 0.01, **** P < 0.0001.

Journal: bioRxiv

Article Title: Single-cell atlas reveals age-related cellular shifts underlying fibrosis in murine synovium

doi: 10.64898/2026.04.17.719179

Figure Lengend Snippet: a , Heatmap showing expression of top 20 genes across synovial macrophage clusters, identifying Pf4 + , Vsig4 + and Cd74 + macrophage subsets. b , Violin plots depict expression of representative marker genes across the three macrophage subsets. c , Gene Ontology enrichment analysis of genes upregulated in young versus aged macrophages within each subset. Dot size indicates gene ratio and color indicates adjusted P value. d , IL-6 and TNF-α secretion by FACS-sorted synovial macrophages from young and aged mice following 24 h ex vivo stimulation with or without LPS (n = 10 mice per group; one-way ANOVA test). e , Phagocytosis assay showing representative pHrodo Green fluorescence histograms and frequency of pHrodo + cells within CD64 + F4/80 + synovial macrophages, 2 h after intra-articular injection of pHrodo Green E. coli BioParticles (n = 6 mice per group; two-tailed Mann-Whitney U test). FMO control is shown in grey. Data are mean ± s.e.m. ** P < 0.01, **** P < 0.0001.

Techniques: Expressing, Marker, Ex Vivo, Phagocytosis Assay, Fluorescence, Injection, Two Tailed Test, MANN-WHITNEY, Control

Review

human il 6 elisa duoset kit (R&D Systems)

Structured Review

Images

1) Product Images from "PM2.5 toxin benzo[a]pyrene induces life-limiting inflammation and oxidative stress in the airway by up-regulation of TRPC6 and inactivation of β2AR/CFTR signaling"

Related Articles

Enzyme-linked Immunosorbent Assay:

other:

In Vivo:

Cell Culture:

Similar Products

Image Search Results